RESUMO
Objetivo: Evaluar el efecto de una preparación con ribosomas bacterianos y proteoglicanos de Klebsiella pneumoniae «R» sobre la expresión in vitro de las moléculas CD11c y CD103 en neutrófilos de sangre periférica. Métodos: Aislamiento de neutrófilos de sangre periférica con Ficoll-Paque, incubación con R y detección de CD11c y CD103 mediante citometría de flujo. Resultados: A las 6h postincubación, la expresión de CD11c aumentó de manera significativa respecto al control con 125 y 500 microgramos/ml de R (p=0,017 y p=0,006, respectivamente). La expresión de CD103 inducida con 125 microgramos/ml de R a las 6h fue significativamente mayor que la observada a las 4h a la misma concentración (p=0,014) y que la encontrada con 62,5 microgramos/ml (p=0,017). Conclusiones: El aumento en la expresión de CD11c y CD103 inducido por R en neutrófilos podría contribuir al mecanismo de acción de R contra patógenos respiratorios(AU)
Aim: To evaluate the effect of a preparation with bacterial ribosomes and proteoglycans from Klebsiella pneumoniae «R» on the in vitro expression of CD11c and CD103 molecules in neutrophils from peripheral blood. Methods: Isolation of neutrophils from peripheral blood with Ficoll-Paque, incubation with R and detection of CD11c and CD103 through flow cytometry. Results: Six hours after the incubation period, CD11c expression increased significantly compared with the control with 125 and 500 microgramos/ml of R (P=0,017 and P=0,006, respectively). CD103 expression induced with 125 microgramos/ml of R after 6hours was significantly higher than that observed after 4hours at the same concentration (P=0,014) and that found with 62.5 microgramos/ml (P=.017) of R. Conclusions: The increased expression of CD11c and CD103 induced by R in the neutrophils could contribute to the R mechanism against respiratory pathogens(AU)
Assuntos
Humanos , Masculino , Feminino , Gravidez , Recém-Nascido , Lactente , Pré-Escolar , Neutrófilos , Sangue , Ribossomos , Proteoglicanas , Infecções RespiratóriasRESUMO
AIM: To evaluate the effect of a preparation with bacterial ribosomes and proteoglycans from Klebsiella pneumoniae «R¼ on the in vitro expression of CD11c and CD103 molecules in neutrophils from peripheral blood. METHODS: Isolation of neutrophils from peripheral blood with Ficoll-Paque, incubation with R and detection of CD11c and CD103 through flow cytometry. RESULTS: Six hours after the incubation period, CD11c expression increased significantly compared with the control with 125 and 500µg/ml of R (P=.017 and P=.006, respectively). CD103 expression induced with 125µg/ml of R after 6hours was significantly higher than that observed after 4hours at the same concentration (P=.014) and that found with 62.5µg/ml (P=.017) of R. CONCLUSIONS: The increased expression of CD11c and CD103 induced by R in the neutrophils could contribute to the R mechanism against respiratory pathogens.